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Portable Test Quickly Detects Zika Virus in Saliva for $2
Anxiety over the Zika virus is growing as the Olympic Games in Rio de Janeiro approach. To better diagnose and track the disease, scientists at the University of Pennsylvania have developed a $2 test that can accurately detect low levels of the virus in saliva. Their work was reported online in Analytical Chemistry, a journal of the American Chemical Society.
The World Health Organization (WHO) recently announced that there was no need to postpone or move the Olympics because of Zika’s presence, but concern over the virus’ spread and its link to serious birth defects is far from allayed. Public health experts debate whether WHO made the right call. While the discussion continues, scientists are working on new tools to help manage the outbreak.
Current gold-standard tests to detect the Zika virus require expensive laboratory equipment and trained personnel. Low-cost diagnostic methods have been reported, but they can’t detect low levels of the disease or distinguish between Zika and similar viruses, such as dengue.
The scientists at Penn wanted to design a rapid, low-cost, and more-reliable point-of-care detection test. Their efforts resulted in a so-called reverse-transcription loop-mediated, isothermal amplification (RT-LAMP) assay. The assay is implemented in an easy-to-use, inexpensive, point-of-care disposable cassette that carries out all of the unit operations from sample introduction to detection. For thermal control of the cassette, the scientists used a chemically heated cup without a need for electrical power.