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Scientists Adopt New Strategy to Find Huntington’s Disease Therapies

NIH-supported study uses precision medicine to tackle neurological disorders

Scientists searched the chromosomes of more than 4,000 Huntington’s disease patients and found that DNA repair genes may determine when the neurological symptoms begin. Funded in part by the National Institutes of Health (NIH), the results may provide a guide for discovering new treatments for Huntington’s disease and a roadmap for studying other neurological disorders. 

“Our hope is to find ways that we can slow or delay the onset of Huntington’s devastating symptoms,” says James Gusella, PhD, director of the Center for Human Genetic Research at Massachusetts General Hospital (MGH), Boston, and the corresponding author of the study published online in Cell. “This could be possible because we now have a list of clinically proven genetic factors that influence the disease.”

Huntington’s disease is an inherited neurodegenerative disorder caused by mutations in a gene that encodes a protein called Huntingtin. Symptoms usually begin in midlife and include uncontrolled movements, emotional disturbances, and, eventually, dementia. Although studies in humans and animals have discovered clues as to how the disorder works, there are no effective treatments.

For this study, the scientists tried a slightly different approach. They employed a novel application of Genome Wide Association Study analysis, a technique that scientists typically use to search for single-letter changes to the DNA code on patients’ chromosomes, which may increase or decrease their chances of having the disease. Here, the scientists already knew the disease-causing gene, so they used the technique to search for other changes that determine when the disease starts. They found that at least three sites were associated with symptoms appearing earlier or later than expected, two on chromosome 15 and one on chromosome eight.

“This approach could have a significant impact on Huntington’s disease patients and researchers,” says Margaret Sutherland, PhD, program director of the National Institute of Neurological Disorders and Stroke, part of NIH. “It’s an example of how precision medicine may be applied to neurological disorders.”

The study was conducted by the Genetic Modifiers of Huntington’s Disease Consortium, an international team of scientists devoted to finding treatments for the disorder. Starting more than three decades ago, they collected DNA samples and clinical information from patients, mainly in the United States, Canada, and Europe. 

The scientists used the patients’ DNA and clinical information to study the age when movement problems began. Located on chromosome four, the Huntingtin gene is characterized by three-letter repeats in the DNA code, called CAG-repeats. Disease-causing versions of the Huntingtin gene contain more than 35 CAG-repeats, which is higher than the six to 34 repeats found in normal versions. The greater number of CAGs a patient has, the greater the chances that symptoms will appear earlier in life. Focusing on patients who had 40 to 55 CAGs, the scientists found that some developed the disease earlier than expected while others developed it later. 

The scientists then analyzed the patients’ chromosomes using gene chips that contain sites known for having single-letter changes to the DNA code in normal human populations. After multiple rounds of searching, they found that two sites on chromosome 15 were strongly associated with disease onset. One site was associated with hastening the disease by, on average, just over six years; another site was associated with slowing the disease by about a year and a half. They also found that a site on chromosome eight was associated with earlier disease onset by nearly one and a half years. 

Although the scientists did not identify specific genes, they used several types of genome network analyses to show that disease onset may be controlled by genes that repair DNA, catalyze essential chemical reactions in cells, and assist with the division of mitochondria. In addition, they noted that a site within the code for MLH1, a DNA repair gene located on chromosome three, may be involved. Finally, the scientists showed that the sites on chromosomes 15 and eight may be associated with the beginning of psychiatric and cognitive problems caused by the disease.

Source: NIH, August 7, 2015.

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